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Lab Report 1Allison M. ShawBio 302L Sec 009Thursday February 12th, 2014 instaurationHL-60 kiosks were initially taken from a Caucasian female that had acute promyelocytic leukemia (Cirtain, Mittelstadt, Higgins, Pollson, 2002). These cells are good for studying cellular processes and under favorable conditions, are perpetual. HL-60 cells have a relatively quick doubling time of no more than 48 hours, and do not experience apoptosis, which makes them immortal (Cirtain, et. al, 2002). The HL-60 cell lines immortality is instrumental when it comes to finding possible preachings for patients with leukemia, which is of interest to researchers. This immortality impute is also critical for studying mechanisms of monocyte and granulocyte differentiation (Collins, 1987). Another quality well-nigh the HL-60 cell line that is equally measurable is its ability to differentiate into the purity blood cell line (Collins, 1987). Granulocytes and monocytes are the two most important types of differentiated cells in this experimentation, although white blood cells also differentiate into macrophage-like cells and eosinophils (Collins, 1987). Compounds occurring by nature that induce HL-60 cell line differentiation include Vitamin D (Collins, 1987). chemical substance inducers, such as DMSO and PMA in this experiment, can induce HL-60 cells to differentiate into cells on the myeloid path (Cirtain et al,. 2002). DMSO is studied as a control for PMA treatment conditions and is used to make PMA solution. It causes HL-60 cells to differentiate mostly into granulocytes (Cirtain et al,. 2002). The control in this experiment is cells treated with DMSO, and is done to show DMSO has no effect on PMA (Cirtain et al,. 2002). utilize kinase C, PMA activates cert... ...he line. Further studies could allow researchers to gain valuable information possibly tip to cures for numerous cancer types. ReferencesCirtain, Mittelstadt, Higgins, Pollson. Chapter 3. Power Point. Cell and Molecular Biology research lab Manual. Great River Tech. Web. 8 Feb 2014. Cirtain, Mittelstadt, Higgins, Pollson. Chapter 4. Power Point. Cell and Molecular Biology science laboratory Manual. Great River Tech. Web. 8 Feb 2014. Collins, S. J. The HL-60 Promyelocytic Leukemia Cell Line Proliferation, Differentiation, and Cellular Oncogene Expression. The demarcation Journal 70 (1987) 1233-1244. Xie, Bei, Amale Laouar, and Eliezer Huberman. Fibronectin-mediated Cell Adhesion Is Required for Induction of 92-kDa fount IV Collagenase/Gelatinase (MMP-9) Gene Expression during Macrophage Differentiation. The Journal of Biological Chemistry (1998) 1576-1582.